Fig. 5.

ADAM17 knockdown inactivates EGFR signaling and reduces cell proliferation and goblet cell phenotype. a. Immunoblot of ADAM17, pEGFR and EGFR in Caco-2 and LS174T cells transfected with ADAM17-specific siRNAs #1 and #2 or non-silencing siRNA. β-Actin was used as a loading control. b. Time-lapse cell growth assay showing the effect of ADAM17 knockdown by siRNAs on Caco-2 and LS174T cell growth. Bars, mean ± s.d.; n = 3 wells; **, p < 0.01. c. Effect of ADAM17 knockdown by siRNAs on mucus production in LS174T cells. Mucus production was evaluated by Alcian-Blue (AB) staining (left panel). Scale bars, 20 μm. The ratio of AB-positive area in the total cell area is determined (right panel). Bars, mean ± s.d.; n = 4 dishes; **, p < 0.01. d. Effect of ADAM17 knockdown by siRNAs on MUC2 expression in LS174T cells. Relative gene expression of MUC2 was examined by RT-qPCR. Bars, mean ± s.d.; n = 3 dishes; **, p < 0.01. Results between the two independent groups were determined by Student's t-test, and comparisons among three or more groups were determined by one-way ANOVA followed by Bonferroni's post-hoc testing. P values smaller than 0.05 are indicated on respective plots.