Fig. 2.

The ablation of Pgam5 affects lipid metabolism under fasting and cold stress. (a) The triglyceride (TG) and free fatty acid (FFA) levels in the serum of WT and Pgam5 KO mice under a 6-h cold exposure after 12 h of fasting (n = 21). (b) Estimation of TG release from liver using a lipoprotein lipase (LPL) inhibitor, Triton WR-1339. After 12 h of fasting, WT and Pgam5 KO mice were intravenously treated with PBS or Triton WR-1339 and subjected to cold stress. After 4 h of cold exposure, the serum TG level was measured (n = 8). (c) Representative gross image of BAT from WT and Pgam5 KO mice under basal conditions or at 5 h of cold exposure after 12 h of fasting. (d) BAT weight ratio of WT and Pgam5 KO mice at 6-h cold exposure after 12-h of fasting. (e and f) Representative H&E staining of BAT sections from WT and Pgam5 KO mice under the indicated conditions. Scale bars, 100 μm. (g) Several-tissue weight ratio of WT and Pgam5 KO mice at 6 h of cold exposure after 12 h of fasting. (h) Expression of Elovl3, an elongation factor of FFAs in BAT from WT and Pgam5 KO mice under basal conditions or at 6 h of cold exposure after 12 h of fasting was determined via quantitative RT-PCR (n = 4). Data are expressed as the mean ± SEM, **p < 0.01, ***p < 0.001; unpaired Student's t-test (a and d), two-way ANOVA/Bonferroni post-test (g). BAT, brown adipose tissue; iWAT, inguinal white adipose tissue; eWAT, epididymal white adipose tissue.