Figure 4.

(A) siRNA knock‐down of Hsp70 in H9c2 cells in culture. There was a 70% decrease in Hsp70 expression in cells transfected with Hsp70 siRNA. (B) nsPRP attenuate H₂O₂‐induced ROS production via an Hsp70‐sensitive mechanism. H₂O₂ (44 mmol/L, 10 min) induced a powerful release of ROS in H9c2 cells. Cells pretreated (24 h) with nsPRP (0.5 µL of nsPRP in 3 mL cell culture media) and stimulated with H₂O₂ produced less ROS than cells not receiving nsPRP. However, this effect was partially reversed in cells transfected with Hsp70 siRNA. All data are expressed as means ± SEM. Data are representative of four experimental repeats.