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. 2016 Feb 23;4(4):e12712. doi: 10.14814/phy2.12712

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Changes in viability (panel A1, A2), body mass (panel B1, B2), and egg water loss (panel C1, C2) in d15 embryos stored as eggs for 3 weeks at 22 and 15°C in six replicates of storage procedure. Viability of embryos in each group of 22 and 15°C storage procedures is percent ratio of number of viable embryos (shown in Table 2) to 30 eggs. Body mass is mean value of viable embryos and water loss is mean value of 30 eggs. The different symbols in panels A1, B1, and A2 indicate significant difference between mean values of storage durations (0 (nonstorage), 1, 2, and 3 weeks). When the interaction between storage temperature and storage duration was not significant, the significance symbols are shown only in a 22°C panel. Mean values ± 1 SEM are plotted in panels B1, B2, C1, and C2.