Table 1. Nucleotide sequences of primers used to amplify the C-terminal of KCNE2.

Primer	Sequence (5’-3’)	Ta (°C)
KCNE2-forwardNde1	5’ - ACTGCAGAACATATGCTCAAATCCAAGAGACGG - 3’	50
KCNE2-reverseEcoR1	5’ - ACTGCAGAAGAATTCCTATCAGGGGAACATTTTGAAC - 3’	51

°C: degrees Celsius; Ta: annealing temperature; KCNE2: potassium voltagegated ion-channel subfamily E member 2. The bold text represents a tag, which facilitates restriction enzyme digestion, while the underlined sequences correspond to the Nde1 and EcoR1 restriction enzyme sites, respectively. The short italic sequence (CTA) symbolises the stop codon, and the remaining text represents the sequence of the primer, which will anneal to the DNA in the PCR amplification reaction.