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. 2016 Mar 24;32(1):24–33. doi: 10.5625/lar.2016.32.1.24

Table 1. Primer sequences for qRT-PCR.

Target genes Forward primer (5'→3') Reverse primer (3'→5')
IL-1β CCC TGC AGC TGG AGA GTG TGG TGT GCT CTG CTT GAG AGG TGC T
IL-6 CGA GCC CAC CAG GAA CGA AAG TC CTG GCT GGA AGT CTC TTG CGG AG
iNOS GAT TCA GTG GTC CAA CCT GCA CGA CCT GAT GTT GCC ACT GTT
Cox-2 CCA GAG CAG AGA GAT GAA ATA CCA GCA GGG CGG GAT ACA GTT C
TNF-α CCA GGA GAA AGT CAG CCT CCT TCA TAC CAG GGC TTG AGC TCA
GAPDH AAC GGC ACA GTC AAG GCT GA ACG CCA GTA GAC TCC ACG ACA T

IL-1β: interleukin-1β; IL-6: interleukin-6; iNOS: inducible nitric oxide synthase; Cox-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-alpha; and GAPDH: glyceraldehydes-3-phosphate dehydrogenase.