Table 1. Primer sequences for qRT-PCR.
Target genes | Forward primer (5'→3') | Reverse primer (3'→5') |
---|---|---|
IL-1β | CCC TGC AGC TGG AGA GTG TGG | TGT GCT CTG CTT GAG AGG TGC T |
IL-6 | CGA GCC CAC CAG GAA CGA AAG TC | CTG GCT GGA AGT CTC TTG CGG AG |
iNOS | GAT TCA GTG GTC CAA CCT GCA | CGA CCT GAT GTT GCC ACT GTT |
Cox-2 | CCA GAG CAG AGA GAT GAA ATA CCA | GCA GGG CGG GAT ACA GTT C |
TNF-α | CCA GGA GAA AGT CAG CCT CCT | TCA TAC CAG GGC TTG AGC TCA |
GAPDH | AAC GGC ACA GTC AAG GCT GA | ACG CCA GTA GAC TCC ACG ACA T |
IL-1β: interleukin-1β; IL-6: interleukin-6; iNOS: inducible nitric oxide synthase; Cox-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-alpha; and GAPDH: glyceraldehydes-3-phosphate dehydrogenase.