Figure 2. Detection of leptin receptor mutation by the tetra-primer ARMS-PCR analysis. A. Design of PCR products. Leptin receptor mutation (268 bp, Lepr-FO and Lepr-RI), wild type (199 bp, Lepr-FI and Lepr-RO), internal control from both alleles (407 bp, Lepr-FO and Lepr-RO). B. Genotyping of leptin receptor mutant mice by the tetra-primer ARMS-PCR. C. Genotyping of double knock-out mice by the tetra-primer ARMS-PCR. M: 100 bp ladder.
