Skip to main content
. 2016 Apr 1;6:23187. doi: 10.1038/srep23187

Figure 4. Total number and density of DAPI- and NeuN-positive cells in the GCL and optic nerve axons in glaucoma and ONC eyes.

Figure 4

The upper panel contains graphs presenting exact quantitative analysis and statistical comparisons. SC therapy showed neuroprotective activity towards RGC when compared with control PBS injection. A single asterisk indicates statistical significance at the level of p < 0.05, double asterisk p < 0.01 and triple asterisk p < 0.001. (A–I, M–U) – immunostainings of whole mounted retinas showing GCL cells positive for NeuN with visible reduced cells density within PBS-treated retinas (A–F in glaucoma group; M–R in ONC group) and β3tubulin with marked lower density and thinning of RGC axons (G–I in glaucoma group and S–U in ONC group). (J–L), (V–X) – optic nerve cross-sections in glaucoma and ONC; optic nerves from PBS-treated groups showed severe changes including gliosis, axons swelling and macrophages infiltration, while SC-treated groups presented only mild changes in glaucomatous optic nerves and moderate injury after ONC. Scale bar = 50 μm (D–F), (P–R); 500 μm (A–C), (M–O), (G–I), (S–U); 10 μm (J–L), (V–X) SC – Schwann cells; ONC – optic nerve crush.