Table 2.
Peptides corresponding to SUMO target sites.
| Protein | Isoform specificity | Modified K | Peptide sequence (wt) | Peptide sequence (ctrl) |
|---|---|---|---|---|
| IkBa | S1 | 21 | GPRDGLKKERLL | GPRDGLAKERLL |
| P53 | S1 | 386 | HKKLMFKTEGPD | HKKLMFATEGPD |
| Elk-1 | S1 | 249 | ALPPEVKVEGPK | ALPPEVAVEGPK |
| HDAC4 | S1 | 559 | QAGVQVKQEPIE | QAGVQVAQEPIE |
| ANXA5 | S1 | 29 | TLRKAMKGLGTD | TLRKAMAGLGTD |
| RANBP2 | S1 | 1414 | FALVTPKKEGHW | FALVTPAKEGHW |
| PARP1 | S1 | 203 | KQLPGVKSEGKR | KQLPGVASEGKR |
| HSF1 | S1 | 298 | SPLVRVKEEPPS | SPLVRVAEEPPS |
| NCOA2 | S1 | 258 | GSEVTIKQEPVS | GSEVTIAQEPVS |
| RPS21 | S2 | 41 | NVAEVDKVTGRF | NVAEVDAVTGRF |
| ACIN1 | S2 | 532 | AQPLPLKIEELA | AQPLPLAIEELA |
| HNRNPD | S2 | 197 | PDTPEEKIREYF | PDTPEEAIREYF |
| ANAPC4 | S2 | 772 | GKPVKIKEEVLS | GKPVKIAEEVLS |
| HNRNPC | S2 | 229 | KQAVEMKNDKSE | KQAVEMANDKSE |
| CHD4 | S2 | 1304 | VEREIIKQEESV | VEREIIAQEESV |
| ZMYM4 | S2 | 273 | GLLDKIKDEPDN | GLLDKIADEPDN |
| PCNA | S2 | 164 | VVISCAKDGVKF | VVISCAADGVKF |
| PML | S1+S3 | 490 | CPRKVIKMESEE | CPRKVIAMESEE |
| RPS6 | S3 | 112 | LRASTSKSESSQ | LRASTSASESSQ |
| TRIM28 | S3 | 779 | NKLTEDKADVQS | NKLTEDAADVQS |
| UTP14A | S3 | 733 | HIINPIKAEDVG | HIINPIAAEDVG |
| ZFP106 | S3 | 1265 | EPSQELKFSVEQ | EPSQELAFSVEQ |
| Wiz | S3 | 666 | SPPGTVKAEEHQ | SPPGTVAAEEHQ |
| RPS3A | S3 | 249 | GDETGAKVERAD | GDETGAAVERAD |
| RPS11 | S3 | 249 | LLGETGKEKLPR | LLGETGAEKLPR |
| ZBTB21 | S1+S2 | 430 | VTEVRIKTEPSS | VTEVRIATEPSS |
| HNRNPM | S1+S2 | 698 | KGCGVVKFESPE | KGCGVVAFESPE |
| TRIM24 | S1+S2 | 741 | FPVVIVKQESDE | FPVVIVAQESDE |
| BEND3 | S1+S2 | 20 | LKSITVKVETEA | LKSITVAVETEA |
| NFRKB | S1+S2 | 488 | KDQAFCKQENED | KDQAFCAQENED |
| FOSL2 | S1+S2 | 222 | VGAVVVKQEPLE | VGAVVVAQEPLE |
| PTRF | S1+S2 | 161 | IYQDEVKLPAKL | IYQDEVALPAKL |
| SAFB2 | S1+S2 | 252 | SVGPDRKLAEEE | SVGPDRALAEEE |
| SNIP1 | S1+S2 | 30 | PAGVVVKQERLS | PAGVVVAQERLS |
| TRP120 | S1+S2 | 432 | FNPIVIKEEDKV | FNPIVIAEEDKV |
Amino acid residues in the peptides carrying a lys (K) in the consensus motif are indicated in bold letters. For each peptide, a corresponding negative control sequence is included with alanine (A) substituted in place of K as indicated in bold letter. Wt (Wild-type), ctrl (control).