Figure 1. PUMA deficiency does not affect body weight, glucose or insulin tolerance in mice.

(A) 10 week-old PUMA knockout and wild type male mice were high fat fed for 14 weeks or maintained on a chow diet and body weights determined at the times indicated. (B) Body composition (gonadal white adipose tissue (WAT), liver, gastrocnemius muscle and pancreas relative weights) was determined 17 weeks after high fat feeding. (C) Glucose tolerance tests (2 mg glucose/g body weight; i.p.) were conducted after 16 weeks of high fat or chow feeding and the areas under the curve (AUC) calculated. (D) PUMA deficient and wild type mice after 17 weeks of high fat or chow feeding were subjected to insulin tolerance tests (0.5 mU/g insulin; A.U.C. determined). *p < 0.05, **p < 0.01, ***p < 0.001.