Figure 2. No differences in liver, muscle and adipose tissue insulin sensitivity between high fat fed PUMA knockout and wild type mice.

10 week-old PUMA knockout and wild type male mice were chow-fed or high fat fed for 16 weeks, fasted for 6 h and then injected with PBS or insulin (0.65 mU insulin/g body weight, 10 min). Livers (A), muscle (B) and abdominal white adipose tissue (C) extracted and processed for immunoblotting with the indicated antibodies. The gels have been run under the same experimental conditions and cropped to show protein bands corresponding to p-AKT, AKT, actin or tubulin as indicated. *p < 0.05, **p < 0.01, ***p < 0.001.