Table 1. Primers used in this study.
| Primer Name | Primer Sequence (5′–3′) | Used for |
|---|---|---|
| AjNOS 3-1 | CAGGTTTTTGATGCACGAAATGC | 3′ RACE |
| AjNOS 3-2 | CGCCAATCTCTGGAAGCATCACT | |
| AjNOS 3-3 | CGAGACATTCTGCGTGGGTAAGG | |
| AjNOS 3-4 | CTGACGCTGCTCGGAAAAGGTAG | |
| AjNOS 3-5 | AGCGACCAAGGCAAAATGAGTAT | |
| AjNOS 5-1 | GCACCAAAGATGAGTTCCATTTC | 5′ RACE |
| AjNOS 5-2 | ACTCGCTCTCCTTTCCTTCTACC | |
| AjNOS 5-3 | CCGTAATTCCATGTGTCTCGCTCT | |
| AjNOS 5-4 | TCGGCTAATGTGGTCCTCTGGTT | |
| AjNOS 5-5 | GCCGTTTGAGGAGCCTTATTGAG | |
| Agarginase 3-1 | ACAAGCACGGATTACCAGAAGTC | 3′ RACE |
| Agarginase 3-2 | ACACCAAGCACAGGCACAAGAGT | |
| Agarginase 5-1 | GATTAGGTCTCCTTCCCTTATGG | 5′ RACE |
| Agarginase 5-2 | TGGACTGAAAAATGGGTGACAAC | |
| Ajagmatinase 3-1 | ATGGAATCATCAAAGGGTCTGGA | 3′ RACE |
| Ajagmatinase 3-2 | TTGGTCCGAGGCAAATAAGAACT | |
| Ajagmatinase 5-1 | AATGTCGGCAACTCGTAAGGATG | 5′ RACE |
| Ajagmatinase 5-2 | ATTCCAACAAAGCAGGCATCCAG | |
| AjNOS F | GTAGAAGGAAAGGAGAGCGAGTC | Real-time PCR |
| AjNOS R | CATCGTGTCTCGTCGCATAGTGT | |
| Ajarginase F | AAGCGTTGGGATTCTCGGTGTG | Real-time PCR |
| Ajarginase R | TGGGAGTTCTTCACGAGAGGTTG | |
| Ajagmatinase F | CCTTACGAGTTGCCGACATTGGT | Real-time PCR |
| Ajagmatinase R | CTCGTCAAATGCCCTACGGAATG | |
| Ajβ-actin F | CCATTCAACCCTAAAGCCAACA | Real-time PCR |
| Ajβ-actin R | ACACACCGTCTCCTGAGTCCAT | |
| siRNA interference sequences | AjNOS interference | |
| Sense 1 | GCGACGAGACACGAUGUUUTT | |
| Anti-sense 1 | AAACAUCGUGUCUCGUCGCTT | |
| Sense 2 | GCUGCAGUUCACCGGUAUUTT | |
| Anti-sense 2 | AAUACCGGUGAACUGCAGCTT | |
| Sense 1 | GGACGAUUGUGCCGAUCAATT | Ajarginase interference |
| Anti-sense 1 | UUGAUCGGCACAAUCGUCCTT | |
| Sense 2 | GCGAGAGUACAUGCCACAATT | |
| Anti-sense 2 | UUGUGGCAUGUACUCUCGCTT | |
| Sense 1 | GCGGAUUCAUCGGACGAAATT | Ajagmatinase interference |
| Anti-sense 1 | UUUCGUCCGAUGAAUCCGCTT | |
| Sense 2 | CCAUCAGUGGAUUAGAUAUTT | |
| Anti-sense 2 | AUAUCUAAUCCACUGAUGGTT | |
| Sense | UUCUCCGAACGUGUCACGUTT | Negative control (NC) interference |
| Anti-sense | ACGUGACACGUUCGGAGAATT |