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. 2016 Apr 1;6:23909. doi: 10.1038/srep23909

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Copyright © 2016, Macmillan Publishers Limited

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MIN6 cells were co-immunostained with anti-granuphilin antibody and either anti-Rab27a or anti-Munc18-1 antibody, and were imaged as in Fig. 5. The Rab27a and Munc18-1 clusters associated with granuphilin were further analyzed. Histograms show length of major axis (upper) and ellipticity (ratio of major and minor axes representing cluster shape; lower) of granuphilin-positive Rab27a (blue) and Munc18-1 (green), along with insulin-positive granuphilin (from Fig. 5, red).