FIGURE 7.

Activity of full-length and truncated CjLPMO10A. A, chromatographic analysis of oxidized chito-oligosaccharides from degradation reactions containing 10 mg/ml α-chitin (top chromatograms) or β-chitin (middle chromatograms) after 24 h of incubation with full-length (solid lines) or truncated (dotted lines) CjLPMO10A, with standards for oxidized chito-tetraose (DP4ox) and chito-pentaose (DP5ox). B, MALDI-TOF MS analysis of products generated by the full-length LPMO acting on α-chitin. Prior to the analysis, the samples were saturated with sodium, to simplify the spectra. For each oligomeric product, two major adducts are observed: [DP4–8ox + Na]⁺ and [DP4−8ox−H + 2Na]⁺. All the reactions were carried out in 20 mm Bistris propane buffer, pH 7.2, with 0.5 μm Cu²⁺-saturated LPMO and 1 mm ascorbate as external electron donor.