FIGURE 8.

Synergy between CjLPMO10A and the SmChi18C endochitinase in the degradation of α-chitin. A and B show quantification of GlcNAc and chitobionic acid (GlcNAcGlcNAc1A), respectively, obtained after chitobiase digestion of soluble products from the degradation of 10 mg/ml α-chitin. C, peak areas for chitobionic acid from solubilized material only (gray) and from the complete reaction mixture (black) products after degradation of 2 mg/ml α-chitin using full-length or truncated CjLPMO10A. The total oxidized sugar content was measured after full solubilization of the LPMO-pretreated chitin substrate by the S. marcescens chitinases Chi18A, Chi18C, and chitobiase (CHB). All reactions were carried out at 37 °C in Bistris propane buffer, pH 7.2, in triplicate with 0.5 μm Cu²⁺-saturated LPMO and/or 0.5 μm SmChi18C and in the presence of 1 mm ascorbate as external electron donor.