FIGURE 6.

¹⁴DCCD binding in LHCSR3 recombinant WT and D2E1E2 mutant. Panel A, autoradiography of recombinant LHCSR3 WT and the D2E1E2 mutant treated with ¹⁴DCCD; microliters of sample (0.2 μg/μl of chlorophylls) loaded on SDS-PAGE are reported (15, 7.5, and 2.5 μl). Panel B, Coomassie staining of SDS-PAGE used for autoradiography. Panel C, ratio of the level of ¹⁴C observed by autoradiography signals and protein quantity obtained by densitometric analysis of Coomassie-stained gels. The experiments were performed two times; each time two independent biological replicates were analyzed with three technical replicates with different loading volume as indicated.