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. 2016 Jan 27;291(14):7334–7346. doi: 10.1074/jbc.M115.704601

TABLE 3.

Pigment analysis of recombinant LHCSR3 proteins

Pigment analysis were performed by HPLC and fitting of absorption spectrum of pigment acetone extracts with chlorophylls and carotenoids spectral forms as described in Ref. 34. The experiments were performed two times, with three independent biological replicates each time.

Chl total Chl a Chl b Viola Lute Neo β-car Chl/Car Chl a/b Car total
WT 7 6.29 0.71 0.41 1.56 0.06 0.00 3.38 8.86 2.07
S.D. / 0.10 0.05 0.03 0.07 0.01 0.00 0.31 0.64 0.19
D2E1E2 7 6.51 0.49 0.28 1.63 0.00 0.00 3.61 13.29 1.94
S.D. / 0.09 0.07 0.03 0.05 0.01 0.00 0.22 1.91 0.11