TABLE 5.
Fluorescence lifetimes of LHCSR3 WT and D2E1E2 mutant
The decay traces reported at Fig. 7 were fitting using three exponentials functions. The amplitude (A) and time constants (τ) for each exponential are reported in the table. The average lifetimes for each sample are calculated as ΣAiτi.
| A1 | τ1 | A2 | τ2 | A3 | τ3 | τavg | |
|---|---|---|---|---|---|---|---|
| ns | ns | ns | |||||
| WT, pH 7.5, 0.03% α-DM | 44% | 4.04 | 48% | 1.88 | 8% | 0.21 | 2.69 |
| WT, pH 5, 0.03% α-DM | 40% | 4.22 | 51% | 1.91 | 10% | 0.19 | 2.71 |
| D2E1E2, pH 7.5, 0.03% α-DM | 36% | 4.37 | 54% | 1.93 | 10% | 0.18 | 2.63 |
| D2E1E2, pH 5, 0.03% α-DM | 39% | 4.20 | 52% | 1.83 | 9% | 0.23 | 2.61 |
| WT, pH 7.5, 0.003% α-DM | 40% | 2.61 | 31% | 1.00 | 29% | 0.14 | 1.39 |
| D2E1E2, pH 7.5, 0.003% α-DM | 41% | 2.65 | 30% | 0.98 | 29% | 0.14 | 1.42 |
| WT, pH 5, 0.003% α-DM | 25% | 2.50 | 33% | 0.84 | 42% | 0.14 | 0.97 |
| D2E1E2, pH 5, 0.003% α-DM | 41% | 2.63 | 30% | 0.99 | 29% | 0.14 | 1.41 |