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. 2016 Feb 2;291(14):7373–7385. doi: 10.1074/jbc.M115.710186

FIGURE 7.

FIGURE 7.

Angptl2-induced macrophage migration is mediated by NF-κB and ERK activation. A–D, Transwell assays using peritoneal macrophages (A and B) and RAW264.7 cells (C and D). Compared with WT-derived macrophages, KO-derived macrophages showed lower migration indices with or without additional Angptl2 (A). The migration index was increased by Angptl2 both in the WT-derived peritoneal macrophages (A and B) and RAW264.7 cells (C and D). The increase was significantly suppressed either by DHMEQ or U0126 treatment both in the WT-derived peritoneal macrophages (B) and RAW cells (C). D, either transfection of RelA siRNA or co-transfection of Mapk1 and Mapk3 siRNAs suppressed Angptl2-induced migration in RAW 264.7 cells. E and F, absence of any changes from either DHMEQ or U0126 treatment in the MTT assay compared with controls is shown. n = 8. **, p < 0.01.