FIGURE 5.

Qualitative cellulose binding assays. A, B, and C, binding to Avicel (5% (w/v)), PASC (1% (w/v)), and BMCC (0.35% (w/v)), respectively. Lane a, starting material; lane b, non-bound material in the supernatant after the cellulose had been pelleted; lanes c and d, wash (lane c) and material eluted from washed and pelleted cellulose by boiling in 10% SDS (lane d). Experiments were carried out on ice using 80 μg of proteins in 200 μl of 20 mm sodium phosphate buffer, pH 8.0. 10 mm EDTA was added to the samples containing CfLPMO10_CD and TbLPMO10_CD to prevent any catalytic activity.