TABLE 2.
Binding of CBMs to cellulose
Measurements were performed in 50 mm sodium phosphate buffer, pH 6.0.
| Saturation | Kd | |
|---|---|---|
| μmol/g of cellulose | μm | |
| PASC | ||
| TbCBM2 | 14.73 ± 3.36 | 1.61 ± 0.72 |
| CfCBM2 | 29.58 ± 3.24 | 5.83 ± 1.16 |
| CtCBM3a | 12.32 ± 1.48 | 3.21 ± 1.17 |
| CjCBM10 | 47.14 ± 2.67 | 17.34 ± 3.54 |
| Avicel | ||
| TbCBM2 | 4.44 ± 1.16 | 3.16 ± 2.33 |
| CfCBM2 | 9.94 ± 3.21 | 7.29 ± 3.63 |
| CtCBM3a | 2.25 ± 1.06 | 3.33 ± 1.77 |
| CjCBM10 | 12.93 ± 0.93 | 7.49 ± 1.47 |
| BMCC | ||
| TbCBM2 | 17.03 ± 1.73 | 1.81 ± 0.82 |
| CfCBM2 | 25.29 ± 5.18 | 3.47 ± 1.40 |
| CtCBM3a | 13.76 ± 0.20 | 2.75 ± 0.26 |
| CjCBM10 | 45.72 ± 6.07 | 11.41 ± 1.80 |