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CERS2–6 are phosphorylated. HEK 293T cells were transfected with a plasmid encoding WT HA-CERS1–6 or MT HA-CERS2–6, as indicated. Total membrane fractions prepared from the transfected cells were treated with or without λPPase and PNGase F as indicated, separated by SDS-PAGE, and subjected to immunoblotting with anti-HA antibodies.
