FIGURE 4.

Binding selectivity of the highest affinity CLL 014 ligands. Biotin-tagged KMS30–32 or a control molecule not reactive with CLL 014 IgG was immobilized on streptavidin-coated ELISA plates and titrated with CLL 014, CLL 169, or CLL 068 monoclonal IgGs or a mixture of non-CLL human IgGs. None of the other antibodies represent the subset 7P to which the CLL 014 IgG belongs. The structures of the molecules are shown on the left and the binding curves on the right. Data analysis and curve fitting were performed with GraphPad Prism 6.0 using a nonlinear regression method. Error bars indicate the standard deviation of data obtained from three independent experiments.