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. 2016 Feb 5;291(14):7558–7570. doi: 10.1074/jbc.M115.701656

TABLE 1.

Binding affinities and association and dissociation kinetic parameters determined by ELISA and Biolayer interferometry assay (Octet) for synthetic ligands (KmS31 and KmS32) against CLL 014 BCR Ig

Ligands ELISA KD Octet
kon koff KD
nm 1/ms 1/s nm
KmS31 67 ± 13 2.44 × 104 1.25 × 10−3 51 ± 11
KmS32 167 ± 39 7.98 × 103 1.13 × 10−3 141 ± 27