FIGURE 2.

Cellular FLIPs activate vFLIP binding-deficient IKKγ. 1.3E2, 70Z/3, and 1.3E2 cells stably expressing IKKγ variants were transduced with lentivectors encoding cFLIP variants, vFLIP, and Tax. 48 h post-transduction, cells were treated with the proteasome inhibitor MG-132 (5 μm) for 30 min and then lysed in cell lysis buffer. The cytoplasmic extracts were analyzed for NF-κB activation by immunoblotting with an antibody that recognized phospho-IκBα.