FIGURE 2.

Characterization of plant redox status in two independent experiments (Exp1 and Exp2) by determination of lipid hydroperoxide contents (A), GSH/GSSG ratio (B), and Asc/DHA ratio (C). The analyses relied on the ferrous oxidation-xylenol orange assay with absorption measurement at 650 nm (A), fluorescence measurement at an excitation/emission wavelength of 490/520 nm after specific derivatization using the Amplite Fluorimetric Glutathione GSH/GSSG Ratio Assay kit (B), and the ascorbate oxidase method using detection of DHA absorption at 265 nm (C). Data are means with error bars representing S.D.