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. 2016 Feb 3;291(14):7637–7650. doi: 10.1074/jbc.M115.701417

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effect of phosphorylation on the interaction of Nedd4 WW domains with Cx43CT. A, example of an NMR titration experiment. ¹⁵N HSQC spectra of Nedd4 WW2 alone (black) and in the presence of various molar ratios of Cx43CT^274–288(Ser(P)²⁷⁹, Ser(P)²⁸²) (indicated in the lower right corner) in 1× PBS at pH 7.5 were collected. B, K_D (±S.D.) for the [¹⁵N]WW2/Cx43CT^274–288(Ser(P)²⁷⁹, Ser(P)²⁸²) interaction estimated by fitting the changes in chemical shift (according to the formula Δσ = √((Δδ_HN)² + (Δδ_N/5))²) for the indicated WW2 residues as a function of Cx43CT^274–288(Ser(P)²⁷⁹, Ser(P)²⁸²) concentration. C, summary of the K_D (±S.D.) obtained in the presence of differentially phosphorylated Cx43CT peptides.