FIGURE 6.

Decreased cholesterol transport across Lpcat3 KO enterocytes. Lpcat3-Flox/Villin-Cre-ER^T2 and control female mice were treated with tamoxifen. At day 9 post-treatment, primary enterocytes were isolated and incubated with 0.05 μCi/ml of [¹⁴C]cholesterol as well as unlabeled cholesterol (0.5 mg/ml) for 1 h and washed, and then the radioactivity was counted (A). Cells were also incubated with medium supplemented with oleic acid-containing micelles. After 2 h, the medium was collected and radioactivity counted (B). The conditioned medium was subjected to density gradient ultracentrifugation to determine the distribution of secreted cholesterol in different lipoproteins. Fractions were collected from the top and used to measure cholesterol in triplicate (C). Fractions 1 and 2 represent large and small chylomicrons, respectively. Fractions 8–10 represent intestinal HDL. Values are means ± S.D., n = 3, *, p < 0.01.