TABLE 1.
Measurement of PC subspecies by LC-MS/MS
Values are means ± S.D.; n = 5; *, p < 0.05; **, p < 0.01.
| 16:0/16:0 | 16:0/18:2 | 18:0/18:0 | 18:0/18:1 | 18:1/18:1 | 18:1/18:2 | 18:1/18:3 | 18:0/20:4 | |
|---|---|---|---|---|---|---|---|---|
| Plasma (μg/ml) | ||||||||
| Control | 5 ± 1 | 250 ± 51 | 5 ± 2 | 33 ± 7 | 216 ± 20 | 78 ± 10 | 67 ± 11 | 69 ± 9 |
| Lpcat3 KO | 4 ± 1 | 53 ± 9** | 4 ± 1 | 27 ± 6 | 187 ± 39 | 29 ± 5** | 16 ± 3** | 19 ± 3** |
| Enterocyte plasma membrane (ng/mg protein) | ||||||||
| Control | 113 ± 18 | 5663 ± 519 | 92 ± 17 | 81 ± 13 | 4539 ± 634 | 612 ± 75 | 1815 ± 328 | 2203 ± 339 |
| Lpcat3 KO | 164 ± 22* | 2852 ± 331** | 316 ± 57** | 388 ± 41** | 5954 ± 851 | 396 ± 63* | 788 ± 93** | 723 ± 88** |
| Small intestine homogenate (ng/mg protein) | ||||||||
| Control | 163 ± 29 | 7663 ± 990 | 66 ± 11 | 132 ± 19 | 6231 ± 701 | 832 ± 91 | 2123 ± 425 | 1959 ± 271 |
| Lpcat3 KO | 209 ± 42* | 4568 ± 289** | 196 ± 31** | 299 ± 28** | 6602 ± 981 | 501 ± 79* | 932 ± 120** | 559 ± 65** |
| Liver homogenate (ng/mg protein) | ||||||||
| Control | 268 ± 38 | 8172 ± 1076 | 106 ± 19 | 340 ± 55 | 8782 ± 917 | 718 ± 73 | 3324 ± 551 | 2223 ± 385 |
| Lpcat3 KO | 298 ± 27 | 7392 ± 923 | 129 ± 33 | 389 ± 42 | 9021 ± 1102 | 750 ± 90 | 3267 ± 422 | 2390 ± 343 |
| Kidney homogenate (ng/mg protein) | ||||||||
| Control | 815 ± 105 | 2765 ± 370 | 202 ± 32 | 166 ± 26 | 4519 ± 434 | 591 ± 57 | 2538 ± 318 | 1678 ± 220 |
| Lpcat3 KO | 933 ± 87 | 2983 ± 289 | 251 ± 20 | 180 ± 30 | 4705 ± 378 | 606 ± 71 | 2498 ± 263 | 1543 ± 291 |