Analysis of the products synthesized with BRS-B enzyme from sucrose (292 mm) and dextran 1500 g·mol−1 (66.6 mm).
a, HPAEC-PAD chromatograms of enzymatic reaction stopped after 1 min (1) and enzymatic reaction stopped after 8 h (2). Peaks corresponding to glucose (G), fructose (F), leucrose (L), sucrose (S), and isomaltooligosaccharides with a degree of polymerization (DP) of x. b, 1H NMR spectrum (in the anomeric region) of the products synthesized by BRS-B at 500 MHz in D2O. Leu, leucrose; α-d-Glcp, α-d-glucose; β-d-Glcp, β-d-glucose.