FIGURE 4.

Structures of the deacylated LOS from Bm_manB_core, Bm_wbkD, and BmEPR. A, structure of the penta- and tetra-saccharides in CFG format (top) and standard nomenclature (bottom) obtained by deacylation of the LOS from Bm_manB_core. B, structure of the deca-, nona-, and octasaccharides in CFG format (top) and standard nomenclature (bottom) obtained by deacylation of the LOS from BmEPR and Bm_wbkD. The ratio of the different oligosaccharides present in the samples was estimated by integration of selected cross-peaks in the multiplicity-edited ¹H,¹³C HSQC spectrum; the D3b and D3b* resonances were used to estimate the ratio of oligosaccharides with and without residue E, whereas the G4 and G4** resonances were used to estimate the ratio of oligosaccharides with and without residue I.