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Effect of Ca2+ on the ATPase activity. The ATPase activity of GFP-Spf1p was measured as described under “Experimental Procedures” at 28 °C for 20 s in a reaction medium containing 30 μm ATP, 2 mm Mg2+, 0.5 mm EGTA with (+Ca), or without (−Ca) CaCl2 to give 100 μm Ca2+. The data points are the averages from three experiments. Error bars show the standard deviation.
