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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Nat Cell Biol. 2016 Mar 14;18(4):451–457. doi: 10.1038/ncb3325

Figure 4. godzilla knockdown prevents Wingless transcytosis.

Figure 4

a, b, En face views of a 3rd instar wing imaginal disc expressing RNAi against godzilla (marked by GFP co-expression) in the posterior compartment (with engrailed-gal4). Staining for extracellular Wingless shows that godzilla RNAi causes Wingless to accumulate at the apical surface (a) and to become depleted from the basolateral surface (b). c, The same preparation shown in a transverse optical section. This disc is representative of >10 discs from 2 independent experiments. d, Diagram of Godzilla indicating the two conserved residues (His255 and His258) that were mutated to generate a ligase-dead version. e-g, Expression of GFP-tagged ligase-dead Godzilla (Gzl.LD.GFP), phenocopies godzilla knockdown: accumulation of extracellular Wingless at the apical surface (e) and depletion at the basolateral surface (f). Panel g shows a transverse optical section. This disc is representative of >10 discs from 3 independent experiments. Scale bars represent 10 μm.