FIG 2 .

Cellular and viral 2′,5′-phosphodiesterase (PDE) domains. (A) Alignment of known and predicted cellular and viral 2′,5′-PDE sequences. Catalytic motifs [H-Φ-(S/T)-Φ] are indicated by the red and blue boxes. Rat (Rattus norvegicus [Rn]) and mouse (Mus musculus [Mm]) AKAP sequences and human (H) and bat (Bt) coronavirus sequences are shown. (B) Comparison of known features of full-length M. musculus AKAP7γ, MHV NS2, RVA VP3 CTD and lineage C NS4b proteins including nuclear localization sequence (NLS) and PDE domains. PKA-RII-α-BD, a binding domain for regulatory subunit (RII) of cAMP-dependent protein kinase A (37), guanylyltransferase (Gtase), and methyltransferase (Mtase) domains are also indicated (32).