FIG 4 .

Preferential transfer of Siphoviridae between human individuals. (A) Top differentially transferred genes based on CDD annotation in the Pfam database. A total of 302 CDD hits were found in the contigs. The top two domains (P value, <0.05; odds ratio, >4) based on CDD annotation which were most frequently transferred are shown. (B) Comparison of the frequencies of transfer to least 2 of the 3 patients for Siphoviridae contigs versus all others contigs of >3,000 bp. Transfer is defined here as achieving >50% coverage and >5 paired reads, where the two reads in each pair detect the same contig. If transfer is defined as detection of a donor contig during FMT, after FMT, or both in at least one patient, the favoring of Siphoviridae achieves a P value of <0.0001. (C) Comparison of the frequencies of transfer to least 2 of the 3 patients for Siphoviridae contigs versus all other contigs built. (D to G) Contigs annotating as Siphoviridae found in the donors and suggested by sequence data to be transferred to multiple recipients were validated using qPCR. The samples tested and the annotation details were the same as those described for Fig. 3. (D) Contig 100-72. (E) Contig 100-230. (F) Contig 100-176. (G) Contig 66.