Table 3. NOTCH1 genetic variant (rs139994842) linked to somatic mutations in NOTCH1 is associated with betel quid and HNSCC occurrence using logistic regression adjusted age and substances use covariates.
| Subjects | NOTCH1 rs139994842 | Alcohol drinking | Betel quid chewing | Cigarette smoking | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A/G | G/G | r | D’ | OR (95% CI) | p-value | Yes | No | OR (95% CI) | p-value | Yes | No | OR (95% CI) | p-value | Yes | No | OR (95% CI) | p-value | |
| Case-control study group (282 patients and 282 controls) | ||||||||||||||||||
| Control | 8 | 274 | 1.00 | 82 | 200 | 1.00 | 39 | 243 | 1.00 | 149 | 133 | 1.00 | ||||||
| HNSCC | 17 | 264 | 2.34 (1.02–5.46)* | 0.04 | 190 | 92 | 1.46 (0.88–2.42) | 0.21 | 232 | 50 | 22.45 (13.39–37.64) | <.0001 | 243 | 39 | 1.34 (0.77–2.34) | 0.24 | ||
| 3.46 (1.11–10.84)** | 0.03 | |||||||||||||||||
| SMs validation group (128 patients) | ||||||||||||||||||
| Without SMs | 5 | 99 | 1.73 (0.55–5.41)* | 0.37 | 87 | 16 | 5.71 (2.39–13.64) | 0.0013 | 85 | 8 | 31.55 (13.00–76.60) | <.0001 | 84 | 21 | 0.98 (0.38–2.54) | 0.25 | ||
| 1.15 (0.24–5.50)** | 0.85 | |||||||||||||||||
| With SMs | 5 | 19 | 0.63 | 1 | 9.01 (2.69–30.23)* | 0.0003 | 18 | 5 | 2.17 (0.59- 8.03) | 0.35 | 20 | 3 | 32.11 (10.41–99.05) | <.0001 | 18 | 5 | 0.36 (0.05–2.36) | 0.13 |
| 7.74 (1.70–35.31)** | 0.008 | |||||||||||||||||
r: Correlation coefficient between polymorphisms and somatic mutation (P value = 0.0004).
D’: The coefficient of linkage disequilibrium between SMs and rs139994842.
*Adjusted for substances use.
**Adjusted for substances use and age.