PODXL is a pluripotent feature at transcription levels. (A) Boxplot demonstration of high PODXL expression in human pluripotent stem cells (hPSCs). Reanalysis of pluripotent microarray dataset GSE34200. (B) Normalized RNA-seq read counts of PODXL in hPSCs and somatic cells in comparison with pluripotent (POU5F1, SOX2, and NANOG), somatic (LMNA and CDKN1A), and housekeeping genes (GUSB, ACTB, and GAPDH). Based on dataset GSE66798, GSE71645, and GSE59528. (C) Heat map of RNA-seq data for PODXL expression along with selected genes in cells of early human embryos along with hPSC (hESC, H1, H9, and 3RiPSC2), BJ, and keratinocytes (K). Dataset GSE36552 and GSE66798. (D) Western blots of PODXL expression in hPSCs in comparison with human fibroblasts (BJ), T cells (T), keratinocytes (K), bone marrow stromal cells (MSC), and murine ESCs (Em). Antibody, 3D3. PODXL, podocalyxin-like protein.