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. 2016 Feb 17;25(7):556–568. doi: 10.1089/scd.2015.0321

FIG. 4.

FIG. 4.

KLF4 activates PODXL at an early time during reprogramming. (A) RT-qPCR analysis of PODXL in reprogramming cells. BJ cells were used. OSKM, OCT4, SOX2, KLF4, MYC. Average + SD, n = 3. (B) RT-qPCR measurement of PODXL levels in reprogramming BJ cells during the course of reprogramming. Average + SD, n = 3. (C) Western blots of PODXL with 3D3 antibody. BJ cells were transduced with OSKM viruses. Protein for GFP control was harvested on day 3 postviral transduction. (D) RT-qPCR analyses of PODXL levels in BJ cells transduced with viruses indicated. Time points checked are 48 and 72 h post-transduction. (E) Western analysis of BJ cells transduced with lentiviruses indicated. (F, G) Reporter assay of PODXL regulatory sequence in Hela cells transduced with viruses indicated. (H) qPCR analysis of chromatin DNA immunoprecipitated with PODXL antibody. (I) immunocytochemistry of BJ cells transduced with viruses indicated. *p < 0.05; **p < 0.01; ***p < 0.001. SD, standard deviation.