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. 2016 Feb 17;25(7):556–568. doi: 10.1089/scd.2015.0321

FIG. 6.

FIG. 6.

3D3 is cytotoxic to hPSCs. (A) Flow cytometry analyses of detached H9 treated in test tube for 45 min with antibodies indicated. (B) Flow cytometry analyses of H9 in culture in the presence of antibodies in media for 48 h. (C–E) growth curve of iPSCs (C), H9 (D) and Hela cells (E) treated with antibodies indicated. (F) Western analysis of H1 hESCs treated with 3D3 with p21 antibody. Antibody treatments were performed for 48 h at 3 μg/mL. Fresh antibodies were added at 24 and 2 h before protein harvest with media change.