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. 2016 Feb 9;25(7):542–555. doi: 10.1089/scd.2015.0319

FIG. 4.

FIG. 4.

Inflammation-associated morphological stages of microglia activation and proinflammatory cytokine expression in the V-SVZ of aged mice. (A–D) Resting, hyper-ramified, amoeboid, and round morphologies of microglia activation. Iba1 (green), DAPI (blue). Scale bar: 10 μm for (A, B); Scale bar: 5 μm for (C, D). (E) Quantification of the different stages of microglia activation (as in A–D) in the V-SVZ wholemounts of 2-, 6-, 12-, and 24-month-old mice (n = 3 per group). (F). Relative expression of the proinflammatory cytokines TNFα, IL-1β, and IL-6 as measured by quantitative real time-polymerase chain reaction (qRT-PCR) from the V-SVZ of young adult (2–4 months) and aged (12–15 months) mice (n = 4 per group). (G) Relative expression of IL-6 as measured by qRT-PCR from the V-SVZ of 2 (n = 3) and 6 (n = 2)-month-old mice. Error bars = SEM.