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C5aR1 expression is not affected in macrophages from CCR5Δ32-homozygote individuals. Plastic-adherent cells purified from unfrozen peripheral blood mononuclear cells (PBMCs) of two healthy HIV-uninfected and CCR5 WT individuals and two healthy uninfected CCR5Δ32-homozygote individuals were differentiated into MDM. After differentiation, MDM were stained with (a) anti-CCR5 or (b) anti-C5aR1 mAbs. The gray histograms indicate isotype antibody control staining.
