FIG. 2.
The role of ING1b in osteoblast differentiation. ALP activity was measured in pcDNA3.1-IG1b- or SiING1b-transfected C3H10T1/2 mesenchymal stem cells (A) and MC3T3-E1 preosteoblasts (B) after osteoblast differentiation using an ALP activity kit. (C) Western blotting was used to determine the level of Runx2 in MC3T3-E1 preosteoblasts after osteoblast differentiation. (D) The synthesis of collagen type 1 in MC3T3-E1 preosteoblasts after osteoblast differentiation was measured using the Prolagen-C kit. *p < 0.05 versus control. Runx2, runt-related transcription factor 2.