The effects of ING1b overexpression or silencing on the level of PPAR-β/δ and HIF1α. The mRNA and protein expression of PPAR-β/δ (A, B) and HIF1α (B, C) was determined by RT-PCR and Western blotting, respectively. (D) Western blotting was used to measure the protein expression of PPAR-β/δ and HIF1α in SiHIF1-treated MC3T3-E1 preosteoblasts following osteoblast differentiation. *p < 0.05 versus control or SiMock group, #p < 0.05 versus SiMock+SiING1b group. HIF, hypoxia-inducible factor; PPAR, peroxisome proliferator-activated receptor.