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The effects of PPAR-β/δ siRNA on ING1b-mediated osteoblast differentiation. (A) The PPAR-β/δ level in MC3T3-E1 cells was detected by RT-PCR. (B) ALP activity was measured using an ALP activity kit. (C) The synthesis of collagen type 1 was measured using the Prolagen-C kit. (D, E) Runx2 expression was detected by Western blotting in MC3T3-E1 preosteoblasts following osteoblast differentiation. *p < 0.05 versus SiMock group, #p < 0.05 versus SiMock+SiING1b group.
