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. 2015 May 15;9(12):2725–2739. doi: 10.1038/ismej.2015.68

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Copyright © 2015 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Phylogenetic relationships based on the SSU rRNA gene extracted from the whole-genome sequences of Nahant Bay isolates (NB00##) belonging to the OM43 clade of Betaproteobacteria. The grey box highlights strain NB0046 used in additional growth experiments (Figure 5). The tree was inferred from 1350 alignment positions from sequences curated in the ARB software (reference) using the RAxML (maximum likelihood) method. RAxML bootstrap values (1000 replicates) are shown (>80%) on nodes. Neighbor-joining inference method also produced bootstrap values >80% for these nodes. The scale bar indicates substitutions per site. Gammaproteobacteria and Alphaproteobacteria sequences (not shown) were used as outgroups.