Figure 5.

Phylogenetic tree showing all identified transconjugant OTUs for the same plasmid (pKJK5) introduced through three different donor strains (P.putida, Kluyvera sp. and E. coli). Colors of the branches mark different phylogenetic groups. The three donor strains are shown in white letters in the trees. One green heatmap circle around the tree represents the log-transformed relative OTU abundance in the soil reference recipient community. Three heatmap circles in blue and red display the x-fold over- and underrepresentation of the OTU in the respective transconjugal pool in comparison with the abundance in the reference soil sample. Stars mark the shared (present in all three transconjugal pools) and abundant (present at more than 1% relative sequence abundance) transconjugant OTUs, which constitute the core super-permissive community fraction. Sample size was normalized to 30 000 sequences per transconjugal pool.