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. 2014 Oct 10;9(4):871–881. doi: 10.1038/ismej.2014.185

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Copyright © 2015 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Morphology and basic characters of f327. (a and b) Representative images of phage f327. The tilting angles were set at 0° (a) and ±60° (b), respectively. No obvious width change was observed at different tilting angles. (c) A straight phage filament (red, boxed region in panel a) was chosen for width analysis. The phage component was colored in red. (d) Agarose gel electrophoresis analysis of the genomic DNA of phage f327. Lanes: M, DNA marker; 1, f327 genomic DNA treated with RNase A; 2, f327 genomic DNA treated with S1 nuclease; 3, f327 genomic DNA treated with DNase I; 4, f327 genomic DNA treated with HindIII; 5, untreated f327 genomic DNA. (e) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of f327 phage. Lanes: M, protein ladder; 1, purified f327 phage.