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. 2014 Oct 10;9(4):871–881. doi: 10.1038/ismej.2014.185

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Copyright © 2015 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Distribution of f327 or f327-like phages in the Arctic sea ice Pseudoalteromonas population. (a) Location of the sampling sites in the Arctic sea ice of the Canada Basin where the 53 Pseudoalteromonas strains were isolated. The sites where f327 or f327-like phages were detected are indicated by black triangles. (b) Agarose gel electrophoresis analysis of the PCR product of 19 phage gene-containing strains. Lanes 1–20 were BSi20327, BSi20335, BSi20381, BSi20465, BSi20401, BSi20514, BSi20407, BSi20357, BSi20436, BSi20670, BSi20366, BSi20340, BSi20455, BSi20447, BSi20462, BSi20451, BSi20464, BSi20341, BSi20437 and BSi20439, respectively. Strain BSi20670 harboring no phage gene was used as the negative control. (c) Phylogenetic tree of Pseudoalteromonas based on the rpoD sequences. The black triangles represent the 18 phage-containing strains, which are BSi20357, BSi20341, BSi20407, BSi20439, BSi20447, BSi20401, BSi20437, BSi20381, BSi20366, BSi20514, BSi20462, BSi20465, BSi20451, BSi20455, BSi20335, BSi20436, BSi20340 and BSi20464. ‘BSi20' was omitted from the identification numbers in the figure.