Table 1.
Optimized mass spectrometric parameters for the quantification peptides and their SIL-peptide internal standards. The mass spectrometric parameters were optimized using the SIL peptides. Each SIL peptide contains a [13C615N2]-lysine
| Protein | Peptide Sequence | Precursor Ion (m/z) [SIL-Precursor] |
Fragments (m/z) [SIL-Fragments (m/z)] |
Declustering Potential |
Collision Energy |
|---|---|---|---|---|---|
| RDH11 | VVVVTGANTGIGK | 607.9 [611.9] | 818.4, 717.4 [826.5, 725.4] | 75 | 30 |
| ALDH1A1 | ANNTFYGLSAGVFTK | 795.4 [799.4] | 1042.6, 879.5 [1050.6, 887.5] | 120 | 35 |
| ALDH1A2 | ILELIQSGVAEGAK | 714.4 [718.4] | 846.4, 959.5 [854.4, 967.4] | 120 | 30 |