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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Lett. 2015 Sep 1;369(1):229–241. doi: 10.1016/j.canlet.2015.08.021

Figure 4.

Figure 4

OXi8006 induced increase in stress fiber formation and phosphorylation of FAK (pY397), but not microtubule disruption, is mediated by RhoA/RhoA kinase. Monolayers of rapidly growing HUVECs on gelatin-coated glass coverslips were treated with vehicle, pre-treated for 30 min with 10 µM of the ROCK inhibitor Y-27632 (+Y-27632), 100 nM OXi8006 for 2 h (+OXi8006), or pre-treated for 30 min with 10 µM of the ROCK inhibitor Y-27632 and then treated with 100 nM OXi8006 for 2 h (+Y-27632, +OXi8006). Representative confocal images for endothelial cells fixed and stained with (A) Texas red conjugated phalloidin (red, actin), (B) anti-α-tubulin antibody (green, microtubules), (C) anti-FAK pY397 antibody (white, pFAK), and (D) DAPI (blue, nuclei), merged image. Bars, 20 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)